Antioxidant activities and GC-MS profiling of fractions of methanol extract of Andrographis paniculata

This study was intended to investigate the antioxidant activities of different solvent fractions (hexane (HEX), chloroform (CHL), ethylacetate (ETHYL) and methanol (MET) fractions) extract Andrographis paniculata GC-MS profiling most active fraction (MET). The crude fractionated using vacuum liquid chromatography method. Antioxidant activity evaluated total phenolic content (TPC), flavonoid (TFC), capacity (TAC), ferric reducing potential (FRAP) 2, 2-diphenyl-1-picrylhydrazyl (DPPH), 2-azobis-3-ethylbenzothiazoline-6-sulfonate (ABTS), Nitric oxide (NO) radical scavenging abilities. conducted determine phytochemical present in fraction. results indicated that MET possessed significantly (p < 0.05) higher TPC (33.98 ±1.63 mg GAE/g), TFC (15.81 ± 0.9 5mg QUE/g), TAC (21.44 0.29 AAE/g) FRAP (57.87 0.88 Fe2+/g) than other fractions. Also, exhibited highest (ABTS, DPPH NO) abilities all showed abundance presence 5- octadecadienoic-methyl ester hexadecanoic acid-methyl ester. In conclusion, various A. have could be used diseases associated with free radicals.   Key words: paniculata, antioxidant, solvent-fractions, GC-MS.